The One 16S NGS Library Prep Kit

The ONE 16S NGS Library Preparation Kit, including a Library Quantification kit provides a simple and comprehensive NGS workflow, designed to take you from sample to analysis report with minimal hands-on time. It contains all of the reagents necessary to create a ready-to-sequence NGS library in minutes. In a breakthrough kit format, the user simply performs one closed tube qPCR reaction. The variable regions V3/4 are targeted, amplified and adapters added in a single reaction. The quantitative PCR read out simultaneously quantifies each library so they can be pooled precisely. Then a simple bead-clean completes the workflow.

After sequencing, the data can then be loaded on to our cloud solution for rapid analysis. A detailed report is typically returned within 15 minutes.

Kit Components:
Reagents for Library preparation, quantification and bead clean up of 96 samples

Oligo mix for each sample
The ONE qPCR Mastermix

Magnetic beads
Wash buffer
Elution buffer

Library specific qPCR primers
qPCR MasterMix premixed with SYBRgreen
4 x positive control templates to create standard curve
NGS library dilution buffer